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KMID : 0360220180590111056
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Journal of the Korean Ophthalmological Society
2018 Volume.59 No. 11 p.1056 ~ p.1061
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Effects of Valproic Acid on the Survival of Human Tennon’s Capsule Fibroblasts
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Lee See-Eun
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of valproic acid on the survival of cultured human Tenon’s capsule fibroblasts (HTFBs).
Methods: Primary cultured HTFBs were exposed to 0, 0.25, 0.5, and 1.0 mM valproic acid with or without 0, 1.0, 2.5 μg/mL mitomycin C, and incubated for 5 days. Cell survival was assessed using an MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and the degree of apoptosis was assessed by flow cytometry using annexin?V/propidium iodide double staining.
Results: Valproic acid decreased the survival of HTFBs in a dose?dependent manner, and survival was further decreased by adding mitomycin C to valproic acid. Both valproic acid and mitomycin C induced apoptosis of HTFBs. Valproic acid induced less apoptosis than mitomycin C.
Conclusions: Valproic acid decreased the cellular survival of HTFBs and induced apoptosis. The antiproliferative effects of valproic acid were further enhanced by the addition of mitomycin C.
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KEYWORD
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Apoptosis, Fibroblast, Flow cytometry, Mitomycin C, Valproic acid
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